Oligonucleotide
CRAMSN Research Park is a trusted provider of oligonucleotide synthesis services, offering reliable support for custom oligo synthesis and advanced characterization to meet research and clinical needs.Our state-of-the-art lab facility, supported by skilled scientists, allows us to offer custom phosphoramidite synthesis and perform complex conjugations using click chemistry, amide coupling, and thiol-mediated C-S & S-S bond formation.
We have the capability to synthesize 10-40 mer oligonucleotides up to 10 gram scale and perform all modifications. Our dedicated 2500 sq. ft. lab space is equipped with humidity control, storage capacity, ensuring optimal conditions for high-quality results.
Oligo equipments at CRAMSN:
- ÄKTA oligosynt oligonucleotide synthesizer (10 µmol to 12 mmol ) for DNA/RNA synthesizer
- AKTA pure150 purification system
- ÄKTA flux 6 TFF System for concentration, desalting
- benchtop tray lyophilizer
- Agilent LC-MS for oligonucleotide analysis
- Nano spectrophotometer
Synthesis and Purification
- Scale: 10 µmol to 12 mmol
- Size: 10 to 40-mer
- All standard modifications
- Anion/ Cation exchange purification
- Reverse phase HPLC
- Size exclusion
- Desalting
Analysis
- UV quantification
- Purity and identity analysis using LC-MS (TOF) and mass deconvolution
- HPLC with different mobile and stationary phase for purity assessment
Conjugation
- Acid-amine coupling
- Alkyne-azide coupling
- Thiol-maleimide coupling
- S-S and C-S bond formation
Why CRAMSN Oligonucleotide Synthesis Services?
Complex conjugation using click chemistry,amide coupling and thiol mediated C-C & S-S bond
Ion exchange and reverse phase HPLC
Expertise in nucleoside, sugar modifications, base modifications
State-of-the-art drug discovery facility using oligonucleotide modality
CADD Optimization
Synthesis between 10-40-mer oligonucleotide
In-house custom phosphoramidite synthesis
Integrated Services
Advance Toward Formulation and Delivery :
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