Custom Peptide Synthesis Services

CRAMSN Research Park provides end-to-end custom peptide synthesis, covering discovery to manufacturing. Our expertise ranges from simple linear peptides to complex modified structures. We specialize in solid-phase synthesis using both conventional and microwave assisted synthetic methods, solution-phase synthesis, and hybrid approaches for flexible production. Our capabilities include side-chain modifications for peptides up to 40 amino acids, as well as backbone-modified and derivatized peptides, offering tailored solutions for diverse R&D applications.

Synthesis

We leverage advanced, eco-friendly microwave synthesis technology for high-throughput synthesis of complex peptides, including those with hindered unnatural and N-alkylated amino acids. Our state-of-the-art facilities feature cutting-edge peptide synthesizers, including automated microwave systems, ensuring precision and efficiency.

The facility includes a dedicated purification unit with high-end skids, preparative columns, and ion-exchange columns ranging from 10mm to 100mm ID. It is further supported by a lyophilization lab and temperature-controlled storage unit with temperature range of 2 to 8°C.

Peptide Modification

Analytical Capabilities & Infrastructure

• Precise estimation of Purity of peptides

• Characterisation of peptides using LCMS, IR, Circular dichroism, GPC, mass & NMR

• Salt content estimation through HPLC (TFA, Acetate etc.)

• Residual solvent through GC

Purification Technologies

• Flash chromatography

• Reverse phase purification

• Ion exchange chromatography

• Salt exchange technologies

• Preparative HPLC

• lyophilizer

PROTAC Synthesis and Screening Services

CRAMSN Research Park specializes in the synthesis of PROTACs and partial PROTACs for targeted protein degradation and integrated drug discovery, even at a multi-gram scale. PROteolysis-Targeting Chimeras (PROTACs) are hetero-bifunctional molecules that leverage the body’s natural degradation system to selectively eliminate proteins of interest (POI). By overcoming many limitations of traditional small-molecule inhibitors, PROTACs offer a powerful approach to drug discovery.

With deep expertise in PROTAC synthesis and purification, we also provide comprehensive Profiling using advanced analytical techniques, ensuring high-quality, effective molecules for therapeutic development.

PROTACs – Advantages over Conventional Inhibitors

PROTACs represent a revolutionary shift in drug development, overcoming the limitations of traditional small-molecule inhibitors. By leveraging targeted protein degradation, PROTACs enable the elimination rather than just inhibition of disease-causing proteins, offering greater efficacy and durability. This breakthrough approach also expands drug discovery to previously “undruggable” targets, opening new avenues for treating complex diseases beyond the reach of conventional therapies.

PROTAC Synthesis for Targeted Protein Degradation

• Synthesis of PROTACs and structural analogs from milligram to gram scale and beyond

• Flexible and rigid linker development for novel discovery needs

• Expertise in molecular glues and monovalent protein degraders

• Experienced synthetic team to overcome challenges in PROTAC chemistry

• Advanced analytical support for purification and characterization

• Dedicated preparative HPLC systems for high-purity PROTAC production

• Buchi Lyovapor system to enhance stability and minimize degradation

• SFC and HRMS support for precise analysis and optimization

Oligonucleotide Synthesis Services

CRAMSN Research Park is a leading provider of oligonucleotide synthesis services, specializing in oligo synthesis & characterization. Our state-of-the-art lab facility, supported by skilled scientists, allows us to offer custom phosphoramidite synthesis and perform complex conjugations using click chemistry, amide coupling, and thiol-mediated C-S & S-S bond formation.

We have the capability to synthesize 10-40 mer oligonucleotides up to 1-gram scale and perform all standard modifications, with or without backbone modifications. Our dedicated 1500 sq. ft. lab space is equipped with humidity control, storage capacity, ensuring optimal conditions for high-quality results.

Oligo equipments at CRAMSN:

• ÄKTA oligosynt oligonucleotide synthesizer (10 µmol to 12 mmol ) for DNA/RNA synthesizer

• AKTA pure150 purification system

• ÄKTA flux 6 TFF System for concentration, desalting

• benchtop tray lyophilizer

• Agilent LC-MS for oligonucleotide analysis

• Nano spectrophotometer

Synthesis and Purification

• Scale: 10 µmol to 12 mmol

• Size: 10 to 40-mer

• All standard modifications

• Anion/ Cation exchange purification

• Reverse phase HPLC

• Size exclusion

• Desalting

Analysis

• UV quantification

• Purity and identity analysis using LC-MS (TOF) and mass deconvolution

• HPLC with different mobile and stationary phase for purity assessment

Conjugation

• Acid-amine coupling

• Alkyne-azide coupling

• Thiol-maleimide coupling

• S-S and C-S bond formation

Carbohydrate Synthesis

CRAMSN Research Park offers innovative solutions for the custom synthesis, analysis, and GMP manufacturing of complex multi-step carbohydrates. Recognizing the structural complexity of carbohydrates, we provide expertise in developing therapeutic carbohydrate molecules across a variety of classes, including monosaccharides, polysaccharides, iminosugars, carbocyclic sugars, nucleosides, locked nucleic acids (LNA), glycopeptides, and thioglycosides.

Our capabilities extend to complex multi-step synthesis involving both linear and convergent methods, selective protection and deprotection, and controlling selectivity in moisture-sensitive glycosylations. We also specialize in handling triflates, azide transfer, and oxidation reactions. With hands-on experience in glycosyl donors such as S-Methyl and S-Tolyl, we offer comprehensive downstream purification and isolation, even for water-soluble intermediates.

Lab Infrastructure

We have lab-scale operations of up to 100 grams per batch. Our purification infrastructure at the lab scale includes

• Ion exchange chromatography

• Gel permeation chromatograph

Isolation techniques

We have distillation, precipitation, and lyophilization techniques for isolation.

Manufacturing Infrastructure

• Telescoping operations at a commercial scale

• Operation efficiency to remove chromatographic separation and use of simple techniques to limit impurities such as solubility and solvent-based extractions

• C-18 purification (up to 2 Kg of the crude compound)

• Gel Permeation Chromatography (GPC) purification

• Size exclusion chromatography

• Strong Anion Exchange (SAX) purification

Analytical Capability

Less or no chromophore substrates

• Evaporative Light Scattering Detection

• Refractive Index (RI)

• CAD detector

• Low wavelength UV detection

Highly polar substrates

• Range of specialized carbohydrate columns

• Ion exchange chromatography for polar moieties

Analysis of counter ions e.g., Na+, K+, SO42- SO32-

• Ion chromatography by conductivity detection

Characterization of NCE and its impurities by

• LC-MS, LC-Tof instruments for characterization

• 600 MHz Nuclear Magnetic Resonance Spectrometry

HPAI

At CRAMSN Research Park, we are committed to delivering safe, scalable, and phase-appropriate solutions for high-potent drug development. Our state-of-the-art development facility in Hyderabad, India, is designed to handle highly potent small molecules, ensuring precision, containment, and regulatory compliance at every stage.

Our dedicated high-potency API (HPAPI) development lab operates with Occupational Exposure Limits (OEL) as low as 0.1 µg/m³, making us a trusted partner for high-containment drug development. With a systematic approach to managing HPAPIs, we prioritize safety, efficiency, and scalability, ensuring robust process development and seamless technology transfer.

We specialize in handling a variety of potent molecules, including heterocyclic compounds, nucleosides, steroids, and carbohydrates, offering end-to-end solutions from early-stage discovery to commercial-scale manufacturing.

Our Approach for HPAPI handling

Dedicated HPAPI Labs

• Self-equipped high potent chemical development lab with fume hoods (with negative pressure), barrier isolation, balance connected with isolator

• Multi-purpose solids handling suites

• High potent analytical labs with dedicated equipment connected with pass box

• Biometric access control system

• Capable to take up the development of HPAPI/Intermediates with OEL of 0.1µg/m³

• Man-material entry and exit are separated to avoid contamination

• Mist shower facility available for exit

Dedicated High Potent Analytical Lab

• HPLC instrument with UV, DAD and CAD detectors

• Gas chromatography with ALS and headspace injectors

• Analytical balance (placed in Xpert filtered balance system)

• pH meter

• Ultrasound sonicator and rotary shaker

• Zeta sizer

• Isolator with HEPA cartridge for closed handling of the HPAI compounds

• Pass box for sample transfer and atmos bag

• Fume hood chemical handling and mobile phase preparations

Labelled Compounds

At CRAMSN Research Park, we offer labelled compounds for use in drug discovery, pharmacokinetics, pharmacodynamics, and other specialized research applications. The labelled compounds provide invaluable insights into the behavior and mechanisms of drug candidates, enhancing ability to trace and understand molecular pathways.

• We offer stable isotope labels like 13C, 15N, and 18O to enable accurate mass spectrometry and NMR analysis.

• We provide customized labelling services to meet your specific research requirements.

• All labelled compounds are synthesized with rigorous quality control and characterization to ensure purity, stability, and accuracy.

• From small-scale synthesis for research to large-scale manufacturing for clinical studies.

Why Choose CRAMSN Research Park for Labelled Compounds?

• Expertise in Labeling Techniques: Skilled in a variety of labelling methods to suit your project needs.

• State-of-the-art Facilities: Access to advanced instruments for synthesizing, purifying, and characterizing labelled compounds.

• Regulatory Compliance: Ensuring compliance with relevant safety and regulatory standards, including for radio-labelled compounds.

PDC's - peptide drug conjugates

Peptide Drug Conjugates (PDCs) represent a cutting-edge approach in drug development, combining the specificity and targeting ability of peptides with the potency of cytotoxic drugs. At CRAMSN Research Park, we specialize in the development, synthesis, and optimization of PDCs, offering a comprehensive range of services from early-stage discovery to commercial-scale manufacturing.

Our expert team utilizes advanced peptide synthesis, conjugation technologies, and drug delivery systems to develop PDCs that are tailored to meet the specific needs of your research, ensuring targeted delivery and enhanced therapeutic efficacy.

• We offer stable isotope labels like 13C, 15N, and 18O to enable accurate mass spectrometry and NMR analysis.

• We provide customized labelling services to meet your specific research requirements.

• All labelled compounds are synthesized with rigorous quality control and characterization to ensure purity, stability, and accuracy.

• From small-scale synthesis for research to large-scale manufacturing for clinical studies.

Key Capabilities in PDC Development

1. Peptide Synthesis

• Solid-Phase Peptide Synthesis (SPPS): Synthesis of peptides with high purity, including cyclic and linear peptides.

• Customized Peptides: Tailoring peptides to suit specific biological targets or for use as carriers in drug conjugates.

• Peptide Modification: Introduction of modifications like PEGylation, phosphorylation, and glycosylation to improve stability and solubility.

2. Cytotoxic Drug Conjugation

• Targeted Drug Conjugation: Linking potent cytotoxic drugs (chemotherapeutics, immunomodulators, or small molecules) to peptides through stable, cleavable linkers.

• Drug Loading Optimization: Fine-tuning the drug-to-peptide ratio to maximize therapeutic efficacy while minimizing off-target toxicity.

• Cleavable Linkers: Utilizing cleavable linkers (e.g., disulfide bonds, pH-sensitive linkers) for controlled release of the drug at the target site.

Specialized Equipment & Technology for PDC Development

• Advanced automated peptide synthesizers for the large-scale synthesis of peptides with high purity.

• High-Performance Liquid Chromatography (HPLC) and Mass Spectrometry for precise analysis of peptide purity, molecular weight, and characterization of conjugated compounds.

• Controlled reactors for drug conjugation to peptides under optimized conditions, ensuring efficient conjugation and minimal by-product formation.