Oligonucleotide
CRAMSN Research Park is a trusted provider of oligonucleotide synthesis services, offering reliable support for custom oligo synthesis and advanced characterization to meet research and clinical needs.Our state-of-the-art lab facility, supported by skilled scientists, allows us to offer custom phosphoramidite synthesis and perform complex conjugations using click chemistry, amide coupling, and thiol-mediated C-S & S-S bond formation.
We have the capability to synthesize 10-40 mer oligonucleotides up to 10 gram scale and perform all modifications. Our dedicated 2500 sq. ft. lab space is equipped with humidity control, storage capacity, ensuring optimal conditions for high-quality results.
Oligo equipments at CRAMSN
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ÄKTA oligosynt oligonucleotide synthesizer (10 µmol to 12 mmol ) for DNA/RNA synthesizer
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AKTA pure150 purification system
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ÄKTA flux 6 TFF System for concentration, desalting
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benchtop tray lyophilizer
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Agilent LC-MS for oligonucleotide analysis
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Nano spectrophotometer
Synthesis and Purification
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Scale: 10 µmol to 12 mmol
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Size: 10 to 40-mer
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All standard modifications
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Anion/ Cation exchange purification
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Reverse phase HPLC
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Size exclusion
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Desalting
Analysis
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UV quantification
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Purity and identity analysis using LC-MS (TOF) and mass deconvolution
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HPLC with different mobile and stationary phase for purity assessment
Conjugation
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Acid-amine coupling
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Alkyne-azide coupling
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Thiol-maleimide coupling
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S-S and C-S bond formation
Why CRAMSN Oligonucleotide Synthesis Services?
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Complex conjugation using click chemistry,amide coupling and thiol mediated C-C & S-S bond
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Ion exchange and reverse phase HPLC
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Expertise in nucleoside, sugar modifications, base modifications
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State-of-the-art drug discovery facility using oligonucleotide modality
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CADD Optimization
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Synthesis between 10-40-mer oligonucleotide
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In-house custom phosphoramidite synthesis
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Integrated Services
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